Anti-Mouse CD1d FITC

Also known as: CD1.1, Ly-38

RUO: For Research Use Only

FITC anti-mouse CD1d (CD1.1, Ly-38) antibody, Fluoroscein

Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) PE (cat. 12-0452) and 0.5 µg of Rat IgG2b κ Isotype Control FITC (cat. 11-4031) (left) or 0.5 µg of Anti-Mouse CD1d FITC (right). Total viable cells were used for analysis.

Contents: Anti-Mouse CD1d FITC
Catalog Number: 11-0011
Concentration: 0.5 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: 1B1
Host/Isotype: Rat IgG2b, κ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
11-0011-81	50 ug	To view relevant pricing, please select your country and resume browsing.
11-0011-82	100 ug
11-0011-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4031	Rat IgG2b K Isotype Control FITC

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0011	DISCONTINUED - Anti-Mouse CD1d FITC	488	518	FC	RUO
12-0011	Anti-Mouse CD1d PE	488	575	FC	RUO
13-0011	Anti-Mouse CD1d Biotin	N/A	N/A	FC	RUO
14-0011	Anti-Mouse CD1d Purified	N/A	N/A	FA FC IHC IP	RUO
16-0011	Anti-Mouse CD1d Functional Grade* Purified	N/A	N/A	FA FC	RUO
53-0011	Anti-Mouse CD1d Alexa Fluor® 488	488	519	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with β2-m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects β2-m associated CD1d.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 1B1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 1B1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Brossay L, D.Jullien, S. Cardell, B.C. Sydora, N. Burdin, R.L. Modlin, and M. Kronenberg. 1997. Mouse CD1 is mainly expressed on hemopoietic derived cells. J. Immunol. 159: 1216-1224.
Amano M., N. Baumgarth, M.D. Dick, L. Brossay, M. Kronenberg, L.A. Herzenberg, and S Strober. 1998. CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: β2-microglobulin-dependent and independent forms. J. Immunol. 161:1710-1717.
Sydora B.C., L. Brossay, A. Hagenbaugh, M. Kronenberg, and H. Cheroutre. 1996. TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes. J. Immunol. 156: 4209-4216.
Roark J.H., S.-H. Park, J. Jayawardena, U. Kavita, M. Shannon, and A. Bendelac. 1998. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127.
Kawano, T. J. Cui, Y. Koezuka, I. Toura, Y. Kaneko, K. Motoki, H. Ueno, R. Nakagawa, H. Sato, E. Kondo, H. Koseki, and M. Taniguchi. 1997. CD1d-restricted and TCR-mediated activation of Valpha14 NKT cells by glycosylceramides. Science 278:1626-1629.
Brudin N., L. Brossay, Y. Koezuka, S. T. Smiley, M. J. Grusby, M. Gui, M. Taniguchi, K. Hayakawa, M. Kronenberg. 1998. Selective Ability of Mouse CD1 to Present Glycolipids: α-Galactosylceramide Specifically Stimulates Vα14+ NK T Lymphocytes. J. Immunol 161: 271-81.
Szalay G, Ladel CH, Blum C, Brossay L, Kronenberg M, Kaufmann SH. Cutting edge: anti-CD1 monoclonal antibody treatment reverses the production patterns of TGF-beta 2 and Th1 cytokines and ameliorates listeriosis in mice. J Immunol. 1999 Jun 15;162(12):6955-8. (in vivo, PubMed)

Copyright © 2000-2010 eBioscience, Inc.
Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.