Product Information

Contents: Allophycocyanin-Cy7 (APC-Cy7) anti-human CD27
Catalog Number: 10-0279
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
This product is guaranteed for 6 months upon receipt when stored properly.
Clone: O323
Isotype: Mouse IgG1, κ
HLDA No.: IV T-186

Surface staining of normal human peripheral blood cells with anti-human CD27 (O323) PE (left), and APC (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
10-0279	May be discontinued; see superior product APC-Alexa Fluor® 750 APC-Cy7 anti-human CD27	633	760	FC
11-0279	FITC anti-human CD27	488	518	FC
12-0279	PE anti-human CD27	488	575	FC
13-0279	Biotin anti-human CD27	N/A	N/A	FC
14-0279	Affinity Purified anti-human CD27	N/A	N/A	FC
15-0279	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD27	488	670	FC
17-0279	APC anti-human CD27	633	660	FC
25-0279	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD27	488	760	FC
27-0279	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD27 (APC-Cy7 Equivalent)	633	779	FC
56-0279	Alexa Fluor® 700 anti-human CD27 (APC-Cy5.5 Equivalent)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The O323 antibody has been reported for use in flow cytometric analysis. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage and all handling steps. When staining cells with this conjugated antibody, always cover the samples with aluminum foil.

Applications Tested

The O323 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0271    FITC anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 12-0271    PE anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 13-0271    Biotin anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 14-0271    Affinity Purified anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 16-0271    Functional Grade Purified anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 17-0271    APC anti-mouse, human, rat CD27 (clone LG.7F9)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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