Contents: 10X eBioscience Permeabilization Buffer
Catalog Number: 00-8333
Storage Conditions: Store at 4°C. Use within 6 months of receipt. Cautions: Harmful if inhaled, swallowed or by contact. Wear proper protective clothing and gloves. Note: The 10x Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1x working solution.
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Description
eBioscience Permeabilization Buffer, in conjunction with eBioscience Intracellular (IC) Fixation Buffer (Cat. No.
00-8222) or Foxp3 buffers (Cat. No.
00-5521 can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens. These solutions have been specially formulated for reducing non-specific staining of fluorochrome-labelled antibodies and increasing specific fluorescence signal to noise ratios. For a more detailed protocol of intracellular staining for mouse and human cytokines, please see:
http://www.ebioscience.com/ebioscience/appls/FCI.htm.
The Fixation Buffer is used first in this protocol followed by Permeabilization Buffer. Subsequent washing steps, antibody dilutions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer. The final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer.
The Permeabilization Buffer is supplied as a 10X concentrate. The 10X stock should be diluted in distilled water to a 1X solution prior to use. The 1X final working solution contains 0.1% saponin and 0.009% sodium azide.
Applications Reported
The Permeabilization Buffer is supplied as a 10X concentrate. The 10X stock should be diluted in distilled water to a 1X solution prior to use. The 1X final working solution contains 0.1% saponin and 0.009% sodium azide. The 1x Permeabilization Buffer should be used to resuspend and wash the cell pellet (after cells have been fixed). See “Intracellular Staining Protocol” for detailed use information.
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