Product Information

Contents: eBioscience IC Fixation Buffer
Catalog Number: 00-8222
Sizes: 125 ml
Storage Conditions: Store at 4C. Use within 6 months of receipt. Cautions: Harmful if inhaled, swallowed or by contact.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
00-8222	eBioscience IC Fixation Buffer	N/A	N/A	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

eBioscience Intracellular (IC) Fixation Buffer, used in conjunction with eBioscience Permeabilization Buffer (Cat. No. 00-8333), can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens. These solutions have been specially formulated for reducing non-specific staining by fluorochrome-labelled antibodies and increasing signal to noise ratios. For a more detailed protocol of intracellular staining for mouse and human cytokines, see: http://www.ebioscience.com/ebioscience/appls/FCI.htm.

The Fixation Buffer is used first in this protocol followed by Permeabilization Buffer. All washing steps, antibody dilutions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer.

Applications Reported

This solution is at 1X working concentration and contains 4% Paraformaldehyde, which is toxic and suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

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