Product Information

Contents: eBioscience 7-AAD Viability Staining Solution
Catalog Number: 00-6993
Sizes: 2 ml
Formulation: PBS and 0.09% NaN₃
Storage Conditions: Store at 4°C.
LIGHT-SENSITIVE MATERIAL.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
00-6993	7-AAD Viability Staining Solution	N/A	N/A	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

7-AAD (7-amino-actinomycin D) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. 7-AAD can be used in place of PI (propidium iodide). This solution can be used in combination with PE (phycoerythrin) and FITC (fluorescein isothiocyanate) conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions. Fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Applications Reported

For research use only, not for diagnostic or therapeutic use. 7-AAD can be used as a viability probe for methods of nonviable cell exclusion, based on light scatter and uptake of the reagent as detected in FL3. CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.

Applications Tested

This lot of 7-AAD has been tested by flow cytometric analysis. Use at 5 µl (0.25 μg) per test (per million cells) and incubate for 5 minutes before analysis.

References

MC O’Brien, et al. (1995) Cytometry 19:243
I Schmid et al. (1992) Cytometry 13:204