Product Information

Contents: Foxp3 Staining Buffer Set (Fixation/Permeabilization and Permeabilization Buffers)
Catalog Number: 00-5523
Storage Conditions: Store at 4° C. Avoid temperature fluctuations. Use within 6 months of receipt or by the date indicated on the bottle.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
00-5523	eBioscience Foxp3 Staining Buffer Set	N/A	N/A	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The Foxp3 Staining Buffer Set has been formulated and optimized for Foxp3 staining with the FJK-16s, NRRF-30, PCH101, 236A/E7, and eBio7979 monoclonal antibodies.

Please see the following link for Frequently Asked Questions regarding the usage of eBioscience Foxp3 antibodies and staining reagents: http://www.ebioscience.com/ebioscience/Foxp3FAQs.htm.

Components:

eBioscience Fixation/Permeabilization Concentrate (cat. 00-5123): 30 ml. Store at 4°C. Avoid agitation. Please use within expiration date indicated on the bottle. This is a 4X stock solution that must be diluted prior to use with the Fixation/Permeabilization Diluent. Dilute 1 part Concentrate with 3 parts Diluent.
Caution: This solution contains formaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves.
eBioscience Fixation/Permeabilization Diluent (cat. 00-5223): 100 ml. Store at 4°C. The diluent is intented to be used in combination with the Fixation/Permeabilization Concentrate.
eBioscience Permeabilization Buffer (10X) (cat. 00-8333): 100 ml. Store at 4°C. Dilute to 1X with deionized/distilled water and store at 4°C.
Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1X working solution.

Applications Reported

For research use only, not for diagnostic or therapeutic use. These staining buffers are intended to be used with the Foxp3 antibodies FJK-16s, eBio 7979, NRRF-30, 236A/E7 and PCH101.