Contents: Foxp3 Staining Buffer Set (Fixation/Permeabilization and Permeabilization Buffers)
Catalog Number: 00-5523
Storage Conditions: Store at 4° C. Avoid temperature fluctuations. Use within 6 months of receipt or by the date indicated on the bottle.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
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Emit
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Reported Applications |
| 00-5523 |
eBioscience Foxp3 Staining Buffer Set |
N/A |
N/A |
IC Flow
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Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The Foxp3 Staining Buffer Set has been formulated and optimized for Foxp3 staining with the FJK-16s, NRRF-30, PCH101, 236A/E7, and eBio7979 monoclonal antibodies.
Please see the following link for Frequently Asked Questions regarding the usage of eBioscience Foxp3 antibodies and staining reagents:
http://www.ebioscience.com/ebioscience/Foxp3FAQs.htm.
Components:
- eBioscience Fixation/Permeabilization Concentrate (cat. 00-5123): 30 ml. Store at 4°C. Avoid agitation. Please use within expiration date indicated on the bottle. This is a 4X stock solution that must be diluted prior to use with the Fixation/Permeabilization Diluent. Dilute 1 part Concentrate with 3 parts Diluent.
Caution: This solution contains formaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves.
- eBioscience Fixation/Permeabilization Diluent (cat. 00-5223): 100 ml. Store at 4°C. The diluent is intented to be used in combination with the Fixation/Permeabilization Concentrate.
- eBioscience Permeabilization Buffer (10X) (cat. 00-8333): 100 ml. Store at 4°C. Dilute to 1X with deionized/distilled water and store at 4°C.
Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1X working solution.
Applications Reported
For research use only, not for diagnostic or therapeutic use. These staining buffers are intended to be used with the Foxp3 antibodies FJK-16s, eBio 7979, NRRF-30, 236A/E7 and PCH101.
Mouse Cellular Antigens Chart
Human Cellular Antigens Chart