Contents: Foxp3 Fixation/Permeabilization Concentrate and Diluent
Catalog Number: 00-5521
Storage Conditions: Store at 4° C. Avoid temperature fluctuations. Use within 6 months of receipt or date indicated on the bottle.
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The Foxp3 Fixation/Permeabilization Concentrate and Diluent solutions have been formulated and optimized for Foxp3 staining with the FJK-16s, NRRF-30, PCH101, 236A/E7 and eBio7979 monoclonal antibodies.
Please see the following link for Frequently Asked Questions regarding the usage of eBioscience Foxp3 antibodies and staining reagents:
http://www.ebioscience.com/ebioscience/Foxp3FAQs.htm.
Components:
- eBioscience Fixation/Permeabilization Concentrate: 30 ml. Store at 4°C. Avoid agitation. This is a 4X stock solution that must be diluted prior to use with the Fixation/Permeabilization Diluent. Dilute 1 part Fixation/Permeabilization Concentrate with 3 parts Fixation/Permeabilization Diluent.
Caution: This solution contains formaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves.
- eBioscience Fixation/Permeabilization Diluent: 100 ml. Store at 4°C. The diluent is intented to be used in combination with the Fixation/Permeabilization Concentrate.
Applications Reported
For research use only, not for diagnostic or therapeutic use. These staining buffers are intended to be used with the FJK-16s, NRRF-30, 236A/E7, eBio7979 and PCH101 Foxp3 monoclonal antibodies. Also required for proper intracellular staining with these antibodies is the eBioscience Permeabilization Buffer (10X) (cat.
00-8333). Alternatively, all buffers required for staining with these antibodies can be purchased as a set in the Foxp3 Staining Set (Fixation/Perm and 10x Permeabilization) (cat.
00-5523).
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