Contents: eBioscience Brefeldin A Solution
Catalog Number: 00-4506
Storage Conditions: Store at 4°C.
|
|
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 00-4506 |
eBioscience Brefeldin A Solution |
N/A |
N/A |
IC Flow
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
Brefeldin A is an inhibitor of intracellular protein transport. Incubation of cells in culture with Brefeldin A leads to blockade of protein transport to the Golgi complex (GC) and accumulation of proteins in the endoplasmic reticulum (ER). Addition of Brefeldin A during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines. Brefeldin A is effective for enhanced detection of a majority of mouse and human intracellular cytokines; however, it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Monensin in their specific assay system.
For a more detailed protocol, please see
http://www.ebioscience.com/ebioscience/appls/FCI.htm.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The Brefeldin A Solution is supplied at 1000X working concentration in methanol. Prior to addition to the cell cultures, a 1:1000 dilution in the culture media should be made. The final working concentration of the Brefeldin A (at 1x) is 3.0 ug/ml.
Related Products
Cat. 00-4500 eBioscience Cytokine IC Control Cells for MouseCat. 00-4505 eBioscience Monensin SolutionCat. 00-4509 eBioscience Cytokine IC Control Cells for HumanCat. 00-8222 eBioscience IC Fixation BufferCat. 00-8333 eBioscience Permeabilization Buffer(10X)
