Contents: eBioscience Monensin Solution
Catalog Number: 00-4505
Storage Conditions: Store at 4°C.
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
Monensin is an inhibitor of intracellular protein transport. Incubation of cells in culture with Monensin leads to blockade of protein transport to the Golgi complex (GC) and accumulation of proteins in the endoplasmic reticulum (ER). Addition of Monensin during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines. Monensin is effective for enhanced detection of a majority of mouse and human intracellular cytokines; however, it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Brefeldin A in their specific assay system.
For a more detailed protocol, please see
http://www.ebioscience.com/ebioscience/appls/FCI.htm.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The Monensin Solution is supplied at 1000X working concentration. Prior to addition to the cell cultures, a 1:1000 dilution in the culture media should be made. The final concentration of the monensin (at 1x) is 2 uM.
Related Products
Cat.
00-4500 eBioscience Cytokine IC Control Cells for Mouse
Cat.
00-4506 eBioscience Brefeldin A Solution
Cat.
00-4509 eBioscience Cytokine IC Control Cells for Human
Cat.
00-8222 eBioscience IC Fixation Buffer
Cat.
00-8333 eBioscience Permeabilization Buffer(10X)
