Product Information

Contents: eBioscience Cytokine IC Control Cells for Mouse
Catalog Number: 00-4500
Sizes: 1 ml
Storage Conditions: Store at -80°C for up to 6 months. Short term storage at 4°C.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
00-4500	eBioscience Cytokine IC Control Cells for Mouse	N/A	N/A	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The Mouse Cytokine IC Control Cells are splenocytes which have been activated in vitro and subsequently fixed using paraformaldehyde. These cells are recommended for use as positive control cells when establishing the intracellular cytokine staining protocol. The cell population contains IL-2, IL-3, IL-4, IL-10, GM-CSF, IFN-γ expressing cells. The cells are in a special stabilizing buffer and can be stored at -80°C for up to 6 months or at 4°C for up to a month.

Applications Reported

For research use only, not for diagnostic or therapeutic use. These cells have already been fixed with eBioscience IC Fixation Buffer and need to be permeabilized before the addition of fluorochrome-conjugated antibodies. For each test, take 100 µl of the positive control cells and start the Intracellular Cytokine Staining Protocol at step 4 (i.e., washing with eBioscience Permeabilization Buffer, Cat. No. 00-8333).

For Intracellular Cytokine Staining Protocol, see http://www.ebioscience.com/ebioscience/appls/FCI.htm.