Lysis of normal human, mouse, canine, and rhesus peripheral blood. Total viable cells were used for analysis.
Contents: 10X RBC Lysis Buffer (Multi-species) Catalog Number: 00-4300 Formulation: aqueous buffer, no sodium azide Storage Conditions: Store at 2-25°C. Handling Conditions: Use the 1X solution within 1 month of preparation.
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00-4300-54
50 ml
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŪ 647 conjugated products with eFluorŪ 660 conjugated products.
Description
This 10X RBC Lysis Buffer (Multi-species) is specially formulated for optimal lysis of erythrocytes in single-cell suspensions of peripheral blood and hematopoietic tissues such as spleen. This buffer can be used for lysis of human, mouse, rat, canine, and non-human primate samples. 10X RBC Lysis Buffer (Multi-species) contains ammonium chloride, which lyses red blood cells with a minimal effect on lymphocytes when used as instructed.
Applications Reported
For research use only, not for diagnostic or therapeutic use. 10X RBC Lysis Buffer (Multi-species) has been reported for use in flow cytometric analysis, and Cell culture.
Applications Tested
The 10X RBC Lysis Buffer (Multi-species) has been tested on normal human, mouse, rat, canine, and rhesus peripheral blood followed by flow cytometric analysis.
Protocol
To view protocol, please download the TDS by clicking the PDF button above.
