Custom Antibody Panel and Cocktail Development
Flow cytometric immunophenotyping is a well-established quantitative or qualitative approach for diagnostic modeling, assigning lineage and pathologic classifications. eBioscience offers custom antibody cocktails for multiparametric analysis. Our R&D and manufacturing teams will develop, optimize, validate and manufacture an immunophenotyping method that meets your study’s needs. This will result in greater standardization and less variability, guaranteed lot-to lot reproducibility, and better budget control
Three C's to Custom Cocktail Creation
We specialize in developing and manufacturing antibody panels and cocktails. Work with eBioscience to develop a panel or cocktail tested to meet your exact experimental requirements.
Consult
- Project reviewed with client
- Performance criteria established
- Project start and end dates defined
Concoct
- Determine optimal clones and formats
- Formulate, test, optimize and standardize cocktails using cells or tissues as defined by the client
- Validate performance of the cocktail
Construct
- Scale, manufacture, package and deliver from eBioscience’s ISO certified facility
Benefits
Standardization
- Correlate data globally
- Streamline sample preparation
- Improve precision
- Technician to technician variability
- Lab to lab (site to site) variability
- Experiment to experiment variability
- Option to include compensation controls, buffers and protocols.
Quality
- Establish control by setting release criteria
- Manufactured and QC'ed under our ISO13485 certified system
- Reduce risk and variables
Efficiency
- Greater inventory and purchasing control by reducing number of antibody purchases by different groups. Purchase in test units.
- Resolve the data. Each site and division will have identical standards, clones, and formats allowing for an apple to apple comparison.
- Multiple groups need not optimize and validate the same panel.
Sample 11 Color Antibody Cocktail Experiment
Normal human peripheral blood cells were stained with a validated 11-color panel to optimally identify lymphocyte subsets (T cells, B cells, NK cells) and to enumerate naïve, memory and regulatory T cells. Data were acquired on equipment with 405 nm, 488 nm, 532 nm, and 640 nm laser lines. The panel was designed to maximize separation of each lymphocyte subset while minimizing spectral overlap between fluorochromes to maintain sensitivity for detecting rare and dim antigens, such as CD25 and Foxp3.
*Cells were stained with Fixable Viability Dye eFluor 450, followed by surface staining for CD45, CD56, CD3, CD4, CD8, CD45RA, CD45RO, and CD25. Cells were then fixed & permeabilized using the Foxp3/Transcription Factor Staining Buffer Set and intracellularly stained for Foxp3. CD45+, live, single cells in the lymphocyte gate were used for analysis, further gating on CD3+CD8+ or CD3+CD4+ cells were used for analysis of CD45RA vs CD45RO and CD25 vs Foxp3.
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