CellVue® Maroon Cell Labeling Kit

Description: CellVue® dyes are lipophilic dyes that can be used to label the cell membrane for the purpose of identifying and tracking labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy. Mini CellVue® Kits are supplied with one vial of dye stock (1 mM in ethanol) and one vial of labeling vehicle (Diluent C); Midi CellVue® Kits are supplied with two vials of dye stock (1 mM in ethanol) and six vials of Diluent C.

CellVue® Maroon is a far-red fluorescent cell labeling reagent. It is optimally excited at 647 nm and has a peak emission of 667 nm; however, it can be detected equally in filter sets designed for APC, Alexa Fluor® 700, and APC-eFluor® 780 or similar fluorochromes. Therefore, using antibodies conjugated to these fluorochromes in combination with using CellVue® Maroon is not recommended.

References: Gertner-Dardenne J, Poupot M, Gray B, Fournié JJ. Lipophilic fluorochrome trackers of membrane transfers between immune cells. Immunol Invest. 2007;36(5-6):665-85.

Barbier M, Gray BD, Muirhead KA, Ronot X, Boutonnat J. A flow cytometric assay for simultaneous assessment of drug efflux, proliferation, and apoptosis. Cytometry B Clin Cytom. 2004 May;59(1):46-53.