Cell Proliferation Dye eFluor™ 450 is a violet fluorescent dye that can be used to monitor individual cell divisions. This fluorescent dye binds to any cellular proteins containing primary amines, and as cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye. Up to 7 generations may be visualized. Cells labeled with Cell Proliferation Dye eFluor™ 450 may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers, such as the Foxp3 Transcription Factor Staining Buffer Set (cat. 00-5523) or the IC Fixation Buffer (cat. 00-8222) and Permeabilization Buffer (10X) (cat. 00-8333).

Cell Proliferation Dye eFluor™ 450 has a peak excitation of 409 nm and can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™), making it compatible with applications that utilize GFP.

Cell Proliferation Dye eFluor™ 450 is supplied as a lyophilized powder. Each vial may be reconstituted to a stock concentration of 10 mM with 165 μL of anhydrous DMSO; once reconstituted it should be used within 6 months and protected from light and stored at -20°C with dessicant. Avoid freeze-thawing.

Reported Application
Flow Cytometric Analysis