Anti-CD79a PE

Description: The monoclonal antibody HM47 recognizes the cytoplasmic domain of CD79a, also known as mb-1. CD79a is a 47 kDa membrane glycoprotein that associates with CD79b to form the heterodimeric B cell receptor (BCR). This receptor is responsible for B cell signaling resulting in activation, apoptosis or anergy. Expression of CD79a is found throughout development from the earliest pre-B cell to plasma cells. CD79 is expressed on B cell neoplastic cells and some non-B cell malignancies such as AML. Unlike other antibodies that can be masked in B cell neoplastic cells, HM47 is a reliable antibody for recognizing CD79a and therefore B cells.
Because there are several variants of CD79a, the exact location of the epitope may be slightly different but in humans it is typically at amino acids 208-222.
The HM47 antibody reacts to human, mouse, rat, dog, primates (rhesus, chimpanzee, macaque and baboon) pig, guinea rabbit, pig, horse, cow and chicken.

References: Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A. CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol. 2007 Aug;128(2):306-13.

Miller CJ, Genescà M, Abel K, Montefiori D, Forthal D, Bost K, Li J, Favre D, McCune JM. Antiviral antibodies are necessary for control of simian immunodeficiency virus replication. J Virol. 2007 May;81(10):5024-35.

Astsaturov IA, Matutes E, Morilla R, Seon BK, Mason DY, Farahat N, Catovsky D. Differential expression of B29 (CD79b) and mb-1 (CD79a) proteins in acute lymphoblastic leukaemia. Leukemia. 1996 May;10(5):769-73. (HM47, PubMed)