Anti-Canine CD25 eFluor® 660

Description: This P4A10 monoclonal antibody reacts with canine CD25, the 55 kDa interleukin-2 receptor alpha (IL-2Rα) chain. As in humans and mice, CD25 in dogs is expressed by early T and B lineage progenitors as well as by activated mature T and B lymphocytes. CD25 associates with the IL-2R beta (CD122) and gamma (CD132) chains to form the high-affinity IL-2 receptor. On its own, CD25 binds IL-2 with low affinity. Along with CD4 and Foxp3, CD25 is a major marker used to define regulatory T cells.

The P4A10 antibody has been reported to recognize the same epitope as the anti-human CD25 monoclonal antibody ACT-1, but with greater affinity.

References: Bismarck D, Schütze N, Moore P, Büttner M, Alber G, Buttlar HV. Canine CD4(+)CD8(+) double positive T cells in peripheral blood have features of activated T cells. Vet Immunol Immunopathol. 2012 Oct 15;149(3-4):157-66 (P4A10, FC, Pubmed)

Abrams VK, Hwang B, Lesnikova M, Gass MJ, Wayner E, Castilla-Llorente C, Georges GE, Nash RA. A novel monoclonal antibody specific for canine CD25 (P4A10): selection and evaluation of canine Tregs. Vet Immunol Immunopathol. 2010 Jun 15;135(3-4):257-65. (P4A10, FC, WB, Pubmed)

Mizuno T, Suzuki R, Umeki S, Okuda M. Crossreactivity of antibodies to canine CD25 and Foxp3 and identification of canine CD4+CD25+Foxp3+ cells in canine peripheral blood. J Vet Med Sci. 2009 Dec;71(12):1561-8.

Helfand SC, Modiano JF, Nowell PC. Immunophysiological studies of interleukin-2 and canine lymphocytes. Vet Immunol Immunopathol. 1992 Jun;33(1-2):1-16.