Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
FIGURE: 1 / 1
Description: This YCATE55.9 monoclonal antibody reacts with canine CD8 alpha. This cell surface receptor is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). In dogs, CD8 alpha is expressed on thymocytes, a subset of mature T cells, NK cells, and intestinal intraepithelial lymphocytes (IELs). CD8 binds to MHC class I and plays a role in thymocyte development and mature T cell activation.
This monoclonal antibody has been reported to inhibit cytotoxic T cell generation and function in vitro. In addition, this antibody has been reported to be immunosuppressive in vivo.
Applications Reported: This YCATE55.9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This YCATE55.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8a; CD8alpha; CD8beta; fCD8; Leu-2; leu-2a; T-cell surface glycoprotein CD8 alpha chain
Gene Aliases: CD8A
UniProt ID: (Dog) P33706
Entrez Gene ID: (Dog) 403157, (Dog) 483076
Molecular Function: defense/immunity protein immunoglobulin receptor superfamily
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support