Anti-Canine CD34 PE

Description: The 1H6 monoclonal antibody reacts with canine CD34, also known as mucosialin. CD34 belongs to a protein family that also includes endoglycan and podocalyxin. These single-pass transmembrane proteins possess heavily glycosylated extracellular and N-terminal mucin domains. Similar to humans, canine CD34 can be detected on hematopoietic progenitor cells in the bone marrow, peripheral blood and hair follicle, as well as on some populations of mesenchymal stem cells, tumor cell lines, and on vascular endothelia in the adult. CD34 can be used as a marker to isolate cells capable of hematopoietic cell engraftment.

The 1H6 antibody has been reported to work for enriching CD34+ cells.

References: Pascucci L, Mercati F, Gargiulo AM, Pedini V, Sorbolini S, Ceccarelli P. CD34 glycoprotein identifies putative stem cells located in the isthmic region of canine hair follicles. Vet Dermatol. 2006 Aug;17(4):244-51.

Suter SE, Gouthro TA, McSweeney PA, Nash RA, Haskins ME, Felsburg PJ, Henthorn PS. Isolation and characterization of pediatric canine bone marrow CD34+ cells. Vet Immunol Immunopathol. 2004 Sep;101(1-2):31-47. (1H6, CD34+ enrichment)

McSweeney PA, Rouleau KA, Wallace PM, Bruno B, Andrews RG, Krizanac-Bengez L, Sandmaier BM, Storb R, Wayner E, Nash RA. Characterization of monoclonal antibodies that recognize canine CD34. Blood. 1998 Mar 15;91(6):1977-86. (1H6, FC and WB)