eFluor™ 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ∼520 nm . eFluor™ 514 Calcium Sensor Dye, like Fluo-3 and Fluo-4, is a commonly used dye among the visible light-excitable calcium indicators but with increased cellular uptake (even at room temperature) and brightness.

calcium binding affinity: Kd = 232 nM

Molecular Weight: approximately 1100 Daltons
Peak Excitation: 490 nm
Peak Emission: 514 nm

eFluor™ 514 Calcium Sensor Dye should be reconstituted in high-quality, freshly opened DMSO. Recommended concentration: 2-5 mM.
Once reconstituted, it should be protected from light and stored dessicated at -20°C; avoid freeze-thawing.

Reported Application
Flow Cytometric Analysis, Microscopy