Calcein Violet 450 AM Viability Dye

Description: Calcein Violet 450 AM is a membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450. Calcein Violet 450 is optimally excited at 408 nm and has a peak emission of 450 nm. For fluorescent microscopy, Calcein Violet 450 can be detected using the appropriate filter sets. For flow cytometric analysis, it can be excited off of the violet laser line (405 nm) and detected using filters for Pacific Blue®/eFluor® 450 (450/50). Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells.

Molecular weight: 600
Peak excitation: 408 nm
Peak emission: 450 nm

Calcein Violet 450 AM should be reconstituted in high-quality, freshly opened DMSO.
Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time. Avoid freeze-thawing.

References: Prowse AB, Wilson J, Osborne GW, Gray PP, Wolvetang EJ. Multiplexed staining of live human embryonic stem cells for flow cytometric analysis of pluripotency markers. Stem Cells Dev. 2009 Apr 27.

Cole LE, Shirey KA, Barry E, Santiago A, Rallabhandi P, Elkins KL, Puche AC, Michalek SM, Vogel SN. Toll-like receptor 2-mediated signaling requirements for Francisella tularensis live vaccine strain infection of murine macrophages. Infect Immun. 2007 Aug;75(8):4127-37.