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Calcein AM Viability Dye (UltraPure Grade)

GPR: General Purpose Reagents. For Laboratory Use.

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SKU# 65-0853

Cat. No. Size
65-0853-39 1 mg
65-0853-78 20 x 50 ug

Data for Calcein AM Viability Dye (UltraPure Grade).

BALB/c thymocytes were stained with 12.5 nM Calcein AM for 30 minutes at room temperature (left)....View More

  • Data for Calcein AM Viability Dye (UltraPure Grade).

Description: Calcein AM is a membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein. Calcein is optimally excited at 495 nm and has a peak emission of 515 nm. For fluorescent microscopy, Calcein can be detected using the appropriate filter sets. For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor® 488 (530/30). For multicolor flow cytometry, concentrations in the range of 5 – 20 nM are recommended to ensure that compensation can be performed successfully. Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells. If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854).

Molecular weight: 994.86
Peak excitation: 495 nm
Peak emission: 515 nm

Calcein AM should be reconstituted in high-quality, freshly opened DMSO.
Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time. Avoid freeze-thawing.

Reported Applications Flow Cytometric Analysis, Microscopy
TDS Link Download TDS
Related Products
Cat. No. Name Excite Emit Application Reg.
65-0854 Calcein Violet 450 AM Viability Dye FC, IHC GPR
88-8007 Annexin V Apoptosis Detection Kit APC FC RUO
00-6993 7-AAD Viability Staining Solution FC GPR
65-0855 Calcein Blue AM Viability Dye FC, IHC GPR

References: Kajta M, Wójtowicz AK, Mackowiak M, Lason W. Aryl hydrocarbon receptor-mediated apoptosis of neuronal cells: a possible interaction with estrogen receptor signaling. Neuroscience. 2009 Jan 23;158(2):811-22.

Fonseca PC, Nihei OK, Savino W, Spray DC, Alves LA. Flow cytometry analysis of gap junction-mediated cell-cell communication: advantages and pitfalls. Cytometry A. 2006 Jun;69(6):487-93.

Coder DM. Assessment of cell viability. Curr Protoc Cytom. 2001 May;Chapter 9:Unit 9.2.

Gatti R, Belletti S, Orlandini G, Bussolati O, Dall'Asta V, Gazzola GC. Comparison of annexin V and calcein-AM as early vital markers of apoptosis in adherent cells by confocal laser microscopy. J Histochem Cytochem. 1998 Aug;46(8):895-900.

Weston SA, Parish CR. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J Immunol Methods. 1990 Oct 4;133(1):87-97.

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