Anti-BrdU Purified

Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment.

For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (cat. 00-5525) and protocol.

References: Magaud JP, Sargent I, Clarke PJ, Ffrench M, Rimokh R, Mason DY. Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem. 1989 Oct;37(10):1517-27. (Bu20a, FC)

Beisker W, Dolbeare F, Gray JW. An improved immunocytochemical procedure for high-sensitivity detection of incorpo-rated bromodeoxyuridine. Cytometry. 1987;8:235.

Gratzner, D.F., H.G. Pallavicini and M.G. Gray. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.1983 Proc. Natl. Acad. Sci. USA 80:5573.

Dolbeare F, Gratzner HG, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. 1983 Proc Natl Acad Sci USA.80:5573.