Anti-Asialo-GM1 Alexa Fluor® 488

Also known as: asialo ganglio-N-tetraosylceramide

Clone: Polyclonal

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-6507

Cat. No.	Size
53-6507-80	25 ug
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Staining of C57Bl/6 splenocytes with Anti-Mouse NK1.1 PerCP-Cy5.5 (cat. 45-5941) and 0.25 ug of...View More

Description

Description: This rabbit polyclonal antibody reacts with the asialo ganglio-N-tetraosylceramide (asialo-GM1), a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression. The antibody recognizes asialo-GM1 specifically and not other glycolipids, including GM1 and asialo-GM2.

This antibody has been reported to deplete NK cells in mice and hamsters in vivo and exhibits in vitro effects in a variety of species.

Details

Host	Rabbit
Isotype	IgG
Reactivity	Bovine, Hamster, Human, Mouse, Rat
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
16-6507	Anti-Asialo-GM1 Functional Grade Purified			FC, ICC, FA	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
12-0112	Anti-Mouse CD11b PE	488 - 561 nm	575 nm	FC	RUO
16-6507	Anti-Asialo-GM1 Functional Grade Purified			FC, ICC, FA	RUO
17-5971	Anti-Mouse CD49b (Integrin alpha 2) APC	633 - 647 nm	660 nm	FC	RUO
45-5941	Anti-Mouse NK1.1 PerCP-Cyanine5.5	488 nm		FC	RUO

References

References: Bodhankar S, Woolard MD, Sun X, Simecka JW. NK cells interfere with the generation of resistance against mycoplasma respiratory infection following nasal-pulmonary immunization. J Immunol. 2009 Aug 15;183(4):2622-31. (FC)

Du JW, Zhang F, Capó-Aponte JE, Tachado SD, Zhang J, Yu FS, Sack RA, Koziel H, Reinach PS. AsialoGM1-mediated IL-8 release by human corneal epithelial cells requires coexpression of TLR5. Invest Ophthalmol Vis Sci. 2006 Nov;47(11):4810-8.(FC, ICC, human)

Takeichi N, Li XB, Hamada J, Kobayashi H. Age-related decrease of pulmonary metastasis of rat mammary carcinoma by activated natural resistance. Cancer Immunol Immunother. 1990;31(2):81-5.

Stein-Streilein J, Guffee J. In vivo treatment of mice and hamsters with antibodies to asialo GM1 increases morbidity and mortality to pulmonary influenza infection. J Immunol. 1986 Feb 15;136(4):1435-41. (FA)

Young WW Jr, Hakomori SI, Durdik JM, Henney CS. Identification of ganglio-N-tetraosylceramide as a new cell surface marker for murine natural killer (NK) cells. J Immunol. 1980 Jan;124(1):199-201.

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Antigens	Formats
Asialo-GM1	Alexa Fluor 488