Anti-Asialo-GM1 Functional Grade Purified

Description: This rabbit polyclonal antibody reacts with the asialo ganglio-N-tetraosylceramide (asialo-GM1), a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression. The antibody recognizes asialo-GM1 specifically and not other glycolipids, including GM1 and asialo-GM2.

This antibody has been reported to deplete NK cells in mice and hamsters in vivo and exhibits in vitro effects in a variety of species.

References: Bodhankar S, Woolard MD, Sun X, Simecka JW. NK cells interfere with the generation of resistance against mycoplasma respiratory infection following nasal-pulmonary immunization. J Immunol. 2009 Aug 15;183(4):2622-31. (FC)

Du JW, Zhang F, Capó-Aponte JE, Tachado SD, Zhang J, Yu FS, Sack RA, Koziel H, Reinach PS. AsialoGM1-mediated IL-8 release by human corneal epithelial cells requires coexpression of TLR5. Invest Ophthalmol Vis Sci. 2006 Nov;47(11):4810-8.(FC, ICC, human)

Takeichi N, Li XB, Hamada J, Kobayashi H. Age-related decrease of pulmonary metastasis of rat mammary carcinoma by activated natural resistance. Cancer Immunol Immunother. 1990;31(2):81-5.

Stein-Streilein J, Guffee J. In vivo treatment of mice and hamsters with antibodies to asialo GM1 increases morbidity and mortality to pulmonary influenza infection. J Immunol. 1986 Feb 15;136(4):1435-41. (FA)

Young WW Jr, Hakomori SI, Durdik JM, Henney CS. Identification of ganglio-N-tetraosylceramide as a new cell surface marker for murine natural killer (NK) cells. J Immunol. 1980 Jan;124(1):199-201.