Immunoprecipitation and Western Blot

Immunoprecipitation (IP)

Immunoprecipitation (IP) is an enrichment procedure of specific proteins, protein modifications, or protein motifs from a heterogeneous protein sample such as cell lysate. The enrichment occurs through the use of an antibody that commonly recognizes a protein in its native or semi-native format. IP is often followed by a western blot for analysis, but can be followed by other applications as well. IP allows for the detection of proteins of low abundance, protein:protein interactions and protein post-translational modifications (PTMs).

Western Blot (WB)

Western Blot (WB), also referred to as immunoblot, is a common, effective method for determining relative protein or protein-modification levels in a sample. Denatured proteins are separated via SDS-PAGE and transferred to a membrane where the proteins are detected via the use of antibodies. The relative intensity, and location of the protein on the blot, allow for its identification and quantification.

Antibodies

Enhance and detect your targets with validated antibodies for targets in areas such as cancer, apoptosis, cell signaling, adhesion, neuroscience, and stem cells.