NK and CD8 T cells have been shown to share many of the same inhibitory receptors which have become extremely useful in both the functional and phenotypic characterization of these cells types. Among these is the inhibitory molecule, killer cell lectin-like receptor G1 (KLRG1). In humans, KLRG1 has been shown to be expressed at high levels by NK and some CD8 T cells, as well as mast cells and basophils. Similar to mouse, studies have shown KLRG1+ CD8 T cells to be more senescent with less proliferative potential than their KLRG1– counterparts.
Now, the well-established standards for the detection of KLRG1 by flow cytometry are both available from eBioscience: Clones 13F12F2 and 13A2 developed by Dr. Hanspeter Pircher at the University of Freiburg (Voehringer, 2002).
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FC = Flow Cytometry, Intracellular Staining/Flow Cytometry; ELISA = ELISA, ELISPOT; Multiplex = Multiplex Immunoassays; ICC = Immunocytochemistry; IHC = Immunohistochemistry, Immunofluorescence, Microscopy, Imaging, In Vivo Imaging; IHC-F = Immunohistochemical Staining of Frozen Tissue Sections; IHC-P = Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections; FA = Functional Assays, Bioassays, Neutralization, Depletion Studies, Biomolecule Conjugation; IP = Immunoprecipitation; WB = Western Blotting
RUO = Research Use Only; GPR = General Purpose Reagent; ASR = Analyte Specific Reagent. Analytical and performance characteristics are not established; CE = CE-marked reagents