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BrdU Staining Kits for Flow Cytometry

Incorporate Proliferation Analysis

The immunofluorescent staining of incorporated 5-bromo-2’-deoxyuridine (BrdU), a synthetic nucleoside analog of thymidine, and flow cytometric analysis allow for detection of actively cycling cell fractions. BrdU is incorporated into newly synthesized genomic DNA during the S-phase of mitosis. BrdU is then stained with specific Anti-BrdU (clone Bu20A) fluorescently-labeled antibody and analyzed.  The BrdU staining kits offer a simple, robust and flexible means to incorporate proliferation analysis into current immunophenotyping experiments.

CD4 APC Brdu - image 1CD4 APC Brdu - image 2

Following the protocol provided in the BrdU Staining Kit for Flow Cytometry in the FITC format (cat. no.  8811-6600), CD3/CD28-stimulated mouse splenocytes are unlabeled (left) or labeled with BrdU (right) and stained with Anti-BrdU and Anti-Mouse CD4 APC (cat. no. 17-0041).  Total viable cells were used for analysis.

You want simple, flexible kits? Others can't compete.

eBioscience

Competitor

1-step, 1-buffer fix/perm and wash 3-step fix/perm with multiple buffers and washes
4 fluorochrome formats for violet, blue or red lasers 2 fluorochrome formats for blue or red laser
1 protocol temperature throughout assay 2 protocol temperatures required

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15 Item(s)

Set Descending Direction
25 | 50
Name Clone Application Cat. No. Reg.  

Anti-BrdU APC

BU20A FC 17-5071 RUO

Anti-BrdU eFluor® 450

BU20A FC 48-5071 RUO

Anti-BrdU FITC

BU20A FC 11-5071 RUO

Anti-BrdU PE

BU20A FC 12-5071 RUO

Anti-BrdU PerCP-eFluor® 710

BU20A FC 46-5071 RUO

Anti-BrdU Purified

BU20A FC, IHC 14-5071 RUO

BrdU Kit for IHC/ICC Colorimetric

ICC, IHC 8800-6599 RUO

BrdU Kit for IHC/ICC Immunofluorescence eFluor® 570

ICC, IHC 8841-6599 RUO

BrdU Kit for IHC/ICC Immunofluorescence eFluor® 615

ICC, IHC 8842-6599 RUO

BrdU Kit for IHC/ICC Immunofluorescence eFluor® 660

ICC, IHC 8850-6599 RUO

BrdU Staining Kit for Flow Cytometry APC

FC 8817-6600 RUO

BrdU Staining Kit for Flow Cytometry eFluor® 450

FC 8848-6600 RUO

BrdU Staining Kit for Flow Cytometry FITC

FC 8811-6600 RUO

BrdU Staining Kit for Flow Cytometry PE

FC 8812-6600 RUO

BrdU Staining Kit for Flow Cytometry PerCP-eFluor® 710

FC 8846-6600 RUO

15 Item(s)

Set Descending Direction
25 | 50

* = Please click on product link to see all available sizes and pricing.

FC = Flow Cytometry, Intracellular Staining/Flow Cytometry; ELISA = ELISA, ELISPOT; Multiplex = Multiplex Immunoassays; ICC = Immunocytochemistry; IHC = Immunohistochemistry, Immunofluorescence, Microscopy, Imaging, In Vivo Imaging; IHC-F = Immunohistochemical Staining of Frozen Tissue Sections; IHC-P = Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections; FA = Functional Assays, Bioassays, Neutralization, Depletion Studies, Biomolecule Conjugation; IP = Immunoprecipitation; WB = Western Blotting

RUO = Research Use Only; GPR = General Purpose Reagent; ASR = Analyte Specific Reagent. Analytical and performance characteristics are not established; CE = CE-marked reagents