Anti-Actin (muscle) Alexa Fluor® 488

Clone: HHF35

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-6496

Cat. No.	Size
53-6496-80	25 ug
53-6496-82	100 ug
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Immunocytochemistry on fixed and permeabilized C2C12 cells stained with 30 ug/mL Mouse IgG1 Alexa...View More

Description

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.

The HHF35 antibody recognizes human, mouse, rat, bovine, canine and non-human primate actin.

Details

Host	Mouse
Isotype	IgG1, kappa
Reactivity	Bovine, Canine, Guinea Pig, Horse, Human, Mouse, Pig, Rat
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® is a registered trademark of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Immunohistochemical Staining, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunocytochemistry

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
14-6496	Anti-Actin (muscle) Purified			ICC, IHC, WB	RUO

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References

References: Buccoliero AM, Castiglione F, Degl'Innocenti DR, Franchi A, Sanzo M, Cetica V, Giunti L, Sardi I, Mussa F, Giordano F, Genitori L, Taddei GL Pediatric rhabdoid meningioma: a morphological, immunohistochemical, ultrastructural and molecular case study. Neuropathology. 2011 Feb;31(1):59-65.

Ijzer J, Roskams T, Molenbeek RF, Ultee T, Penning LC, Rothuizen J, van den Ingh TS. Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver. Comp Hepatol. 2006 Nov 16;5:7.

Tsukada T, McNutt MA, Ross R, Gown AM. HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues. Am J Pathol. 1987 May;127(2):389-402.(HHF35, mAb characterization)

Tsukada T, Tippens D, Gordon D, Ross R, Gown AM. HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am J Pathol. 1987 Jan;126(1):51-60.(HHF35, mAb characterization)

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