Anti-Acidic Cytokeratin Purified

Description: The monoclonal antibody AE1 recognizes many members of the acidic (Type I) family of cytokeratins. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an α-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these cytokeratins, and their expression can help identify the origin of a neoplasm.

The AE1 monoclonal antibody recognizes 56.5, 50, 50’, 48, and 40 kDa proteins (also known as CK10, 14, 15, 16 and 19) in mouse, human, rat, primate (cynomolgus and rhesus), dog, cat, rabbit and chicken.

References: Sato T, Maeda H, Suzuki A, Shibuya H, Sakata A, Shirai W. Endometrial stromal sarcoma with smooth muscle and glandular differentiation of the feline uterus. Vet Pathol. 2007 May;44(3):379-82. (AE1/AE3, IHC, feline)

Chen SS, Revoltella RP, Papini S, Michelini M, Fitzgerald W, Zimmerberg J, Margolis L. Multilineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems. Stem Cells. 2003;21(3):281-95.(AE1/AE3, IHC, rhesus)

Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8. (AE1/AE3, WB, IHC, PubMed)

Tseng SC, Jarvinen MJ, Nelson WG, Huang JW, Woodcock-Mitchell J, Sun TT. Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies. Cell. 1982 Sep;30(2):361-72.