**Starting now: ebioscience.com products are on thermofisher.com. Ebioscience.com to retire in April. Get details >


MagniSort® Mouse CD49b Positive Selection Kit

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 8802-6862

Cat. No. Size
8802-6862-74 200 tests

Data for MagniSort® Mouse CD49b Positive Selection Kit.

C57Bl/6 splenocytes were unsorted (left) or sorted with the MagniSort® Mouse CD49b Positive...View More

  • Data for MagniSort® Mouse CD49b Positive Selection Kit.
Description

Description: The MagniSort® Mouse CD49b Positive Selection Kit is designed for the magnetic separation of CD49b+ cells by positive selection. It has been optimized for the isolation of CD49b+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD49b antibody and streptavidin-coated magnetic beads. CD49b+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD49b+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136. For mouse strains that do not express NK1.1 antigen such as BALB/c, SJL, AKR, CBA C3H, Anti-Mouse CD335 (NKp46), clone 29A1.4, may be used.

Details
Reactivity Mouse
Components MagniSort® Anti-Mouse CD49b Biotin (cat. MS13-5971): 200 tests, 20 μL/test; store at 2-8°C.
MagniSort® Positive Selection Beads A (cat. PB-6003): 4 mL; store at 2-8°C.
Documentation
TDS Link Download TDS
Related Products
Cat. No. Name Excite Emit Application Reg.
17-5941 Anti-Mouse NK1.1 APC 633 - 647 nm 660 nm FC RUO
MAG-4902 MagniSort® Magnet, 5 mL RUO
12-5971 Anti-Mouse CD49b (Integrin alpha 2) PE 488 - 561 nm 578 nm FC RUO
01-1234 123count eBeads FC GPR
References