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Anti-Human CD133 FITC

Also known as: AC133, Prominin-1

Clone: EMK08

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 11-1339

Cat. No. Size
11-1339-42 100 tests
11-1339-41 25 tests

Data for Anti-Human CD133 FITC.

Staining of normal human peripheral blood cells with Anti-Human CD45 eFluor® 450 (cat. 48-9459) and...View More

  • Data for Anti-Human CD133 FITC.
Description

Description: The EMK08 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites; the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others.

The binding of the EMK08 antibody does not block the binding of another anti-human CD133 antibody, TMP4 (cat. 12-1338) indicating that they recognize distinct epitopes.

Details
Host Mouse
Isotype IgG2b, kappa
Reactivity Human
Conjugate FITC
Laser Blue Laser
Emit 520 nm
Excite 488 nm
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
12-1339 Anti-Human CD133 PE 488 - 561 nm 578 nm FC RUO
17-1339 Anti-Human CD133 APC 633 - 647 nm 660 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
48-9459 Anti-Human CD45 eFluor® 450 405 nm 445 nm FC RUO
11-4732 Mouse IgG2b K Isotype Control FITC 488 nm 520 nm FC, ICC, IHC RUO
References

References: Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice. Chen Q, Khoury M, Chen J. Proc Natl Acad Sci U S A. 2009 Dec 4. (EMK08, FC, PubMed).

O'Brien CA, Pollett A, Gallinger S, Dick JE. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature. 2007 Jan 4;445(7123):106-10.

Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB. Identification of human brain tumour initiating cells. Nature. 2004 Nov 18;432(7015):396-401. Identification of human brain tumour initiating cells. Nature. 2004 Nov 18;432(7015):396-401.

Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG, Olweus J, Kearney J, Buck DW. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood. 1997 Dec 15;90(12):5002-12.


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